Literature summary extracted from

Guo, L.W.; Assadi-Porter, F.M.; Grant, J.E.; Wu, H.; Markley, J.L.; Ruoho, A.E.
One-step purification of bacterially expressed recombinant transducin alpha-subunit and isotopically labeled PDE6 gamma-subunit for NMR analysis (2007), Protein Expr. Purif., 51, 187-197.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.4.35	expression of isoform PDE6 gamma-subunit in Escherichia coli as an intein-fusion with chitin binding domain. Preparation of homogeneous and isotopically labeled protein for NMR analysis	Bos taurus

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.4.35	C68A	expression of isoform PDE6 wild-type and mutant C68A gamma-subunit in Escherichia coli, preparation of homogeneous and isotopically labeled protein for NMR analysis	Bos taurus
3.1.4.35	additional information	expression of isoform PDE6 wild-type and mutant C68A gamma-subunit in Escherichia coli, preparation of homogeneous and isotopically labeled protein for NMR analysis	Bos taurus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.4.35	10000	-	x * 10000, SDS-PAGE, isoform PDE6 gamma-subunit	Bos taurus

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.35	Bos taurus	-	isoform PDE6	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.4.35	expression of isoform PDE6 gamma-subunit in Escherichia coli, preparation of homogeneous and isotopically labeled protein for NMR analysis	Bos taurus

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.1.4.35	retinal rod	-	Bos taurus	-

Subunits

EC Number	Subunits	Comment	Organism
3.1.4.35	?	x * 10000, SDS-PAGE, isoform PDE6 gamma-subunit	Bos taurus

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Release 2023.1 (January 2023)